Henry Neville Gladstone was born and christened at St. Martin in the Fields, Westminster on 28th April 1852.
He was educated at Eton College and King's College, London. He acted as Private Secretary to his father, the Prime Minister, and was a director of P&O and the B I Steamship Co.
He married Hon. Maud Ernestine, daughter of Stuart Rendel, at St George's Hanover Square, in 1878. They did not have any children.
In 1915 he became Lord of the Manor of Hawarden, on the death of his nephew William Glynne Gladstone, and from 1921 made it his home there until he died.
He succeeded his nephew as Lord Lieutenant of Flintshire and he was President of the University College of North Wales, Justice of the Peace for Flintshire and Cheshire and was awarded the honorary degree of Doctorate of Law (LL.D).
He was raised to the peerage as Baron Gladstone of Hawarden in June 1932.
Henry died at Hawarden Castle in 1935, aged 83 and was buried in Hawarden Churchyard.